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Here, the loss of Tff1 typically leads to an inflammatory response followed by the classical dysplasia-adenocarcinoma sequence [26].In line with this, celecoxib, a selective Cox-2 inhibitor and non-steroidal anti-inflammatory drug disturbed the integrity of the adenoma by promoting ulceration and inflammation [26,27].

The non-transformed rat gastric epithelial cell line RGM-1 [45] (kindly provided by Dr. Before scratch-wounding, cultures were incubated in serum-free, supplemented growth medium for 12-15 h.

A polyclonal rabbit antiserum was generated against the synthetic peptide FHPMAIENTQEEECPF representing the C-terminus of mouse Tff1.

This sequence has already been used successfully as antigenic determinant [28].

Results: Surprisingly, Tff1 expression was up-regulated in migratory cells.

No unequivocal signs of the epithelial-mesenchymal transition were detectable in migratory cells.

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